Evaluating cell activation, killing and movement as a function of time

WORKFLOWS PLATFORM

Technology - Workflows

CellChorus provides comprehensive, dynamic analysis of single cells. Other methods of studying cellular activity lack the ability to integrate dynamic cellular behavior with molecular behavior at the single‑cell level. The company’s TIMING™ (Time-lapse Imaging Microscopy in Nanowell Grids) platform applies visual AI to evaluate cell activation, killing and movement as a function of time in order to maximize our understanding of cellular function, state and phenotype.

 

TIMING Workflow

After receiving cryopreserved effector and target cells from customers, CellChorus thaws and conducts cell line viability studies. CellChorus can also run customer‑specific assays to ensure that cells behave in an expected manner. At this point, CellChorus conducts its single‑cell TIMING workflow as follows:

After cell line validation, cells are loaded into nanowell arrays, incubated and imaged with fluorescent microscopy

1. Acquire images from nanowells

After cell line validation, cells are loaded into nanowell arrays, incubated and imaged with fluorescent microscopy.

CellChorus uses visual AI to segment and track cells, and detect and quantify apoptosis and other cell-to-cell interactions

2. Segment and track single cells

CellChorus uses visual AI to segment and track cells, and detect and quantify apoptosis and other cell-to-cell interactions.

Bead-based sensors enable dynamic, time‑series imaging of secreted cytokines or other biomolecules

3. Image secreted biomolecules

Bead-based sensors enable dynamic, time‑series imaging of secreted cytokines or other biomolecules.

Analyzing the TIMING dataset across thousands of cells quantifies differences in cell behavior and interaction

4. Analyze across the TIMING dataset

Analyzing the TIMING dataset across thousands of cells quantifies differences in cell behavior and interaction.

 

Multiomic Workflow

TIMING analysis enables CellChorus to identify cells of interest for further profiling—providing a more comprehensive picture of how your cells behave.

Perform time‑lapse imaging microscopy in nanowell grids (TIMING workflow)

1. Perform TIMING assays

Perform time‑lapse imaging microscopy in nanowell grids (TIMING workflow).

Identify and select cells of interest based on their dynamic behavior as a function of time

2. Identify cells of interest

Identify and select cells of interest based on their dynamic behavior as a function of time.

Identify and select cells of interest based on their dynamic behavior as a function of time

3. Profile cells of interest

Perform molecular profiling such as single‑cell RNA sequencing on cells of interest.

Integrate TIMING data of dynamic cellular behavior with single‑cell molecular profiling data

4. Integrate data

Integrate TIMING data of dynamic cellular behavior with single‑cell molecular profiling data.

 
Cancer Immunology Research paper - Individual Motile CD4+ T Cells Can Participate in Efficient Multikilling through Conjugation to Multiple Tumor Cells

See an example of the CellChorus workflow from Cancer Immunology Research.

Or view other relevant publications.

View Example
 

The CellChorus platform is broadly applicable across a variety of cell types and applications. Whereas life science researchers have benefitted from significant advances in single-cell analysis, CellChorus goes beyond static assaying. With TIMING, life sciences innovators can watch new biology and gain new and unexpected understandings of how cells move, activate, kill and survive.

 

The company’s technology is protected in part by issued patents and pending patent applications, including US/10,746,736.